Dear self,
Bacteria will grow in highly concentrated LB media (enough media for 20 L growths dissolved in 5 L of water) if left in the warm room overnight thereby pushing your start time ahead three hours while you remake, autoclave, and cool said media.
A frustrated me
Dear growth of e. coli containing really important mutant plasmid,
I have grown you (and your little buddies) multiple times. I am good to you. I feed you, I keep you warm, i put you in a big giant carboy so you can swim around with your buddies. I don't even have you make my protein (which I know you don't like all that much) until after you have partied it up and such. Why then are you giving me so much hassle? I really need you to work right now. Now.
No love
Me
No comments:
Post a Comment